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IR
Laser Diode Imaging
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| An Laser Analytics IR diode laser, tunable between about 1530-1620
cm-1, has been coupled to a Spectra-Tech Research IRplan
microscope (15x objective, 10x condenser) equipped with a 0.25 mm2
broad-band MCT detector element and a Cell Robotics programmable
microscope stage. The FTIR instrument is also used to calibrate
the wavelength output from the diode laser by passing the IR beam
through the interferometer to the internal detector. Light from
the IR diode laser is collimated by an off-axis parabolic mirror
and passed through a chopper before entering the microscope. The
transmitted light is collected and focussed onto the detector. The
modulated signal is then passed to a lock-in amplifier, digitized,
and passed to the computer.
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