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Raman Imaging
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| The Integrated Spectroscopy Resource |
Raman Imaging |
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Raman Point Spectra |
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Raman spectrum extracted from 2D Raman image of RBC. |
Left: The spectrum is clearly dominated by hemoglobin bands as expected, but also has contributions from other cellular components. The band near 1365 cm?1 depends on the oxygenation state of the hemoglobin and is typically 1355 cm?1 for the deoxy form, 1379 cm?1 for the oxy form of hemoglobin. | ||||||||||||||||||
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Right: This line image represents a cross section of the RBC. Clearly there is a large decrease in intensity in the middle of the cell, consistent with its biconcave shape. The thickness of RBCs vary from ~1.5 µm near the middle up to ~1.8 µm near the periphery. The edge of the cell also gives an indication of the resolution of the image. Taken together, these observations demonstrate that we are able to observe sub micron level details of cells. |
Spatial (line) image extracted from 2D Raman image of RBC. |
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Operated by the Los Alamos National Security LLC for the US Department of Energy Last Modified: June 5, 2006 |
